Regulatory

Part:BBa_K2715013:Design

Designed by: Daniel Partridge   Group: iGEM18_Nottingham   (2018-10-08)


Native promoter from C. difficile toxin B gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was natively Biobrick compatible and so did not require modification. The -35 and -10 binding regions were predicted using the BPROM algorithm (see references below), and the promoter region identified.


Source

This basic part is the promoter region regulating toxin B expression from the Gram-positive organism Clostridium difficile.